Sprouty Homolog 1 (Protein Sprouty Homolog 1, Spry-1, SPRY1)
Product Sizes
100ug
£876.00
S5620-01D-100UG
About this Product
- SKU:
- S5620-01D
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- SPRY1 (sprouty homolog 1) is a 34-38kD member of the sprouty family of proteins. It is widely expressed, being found in multiple embryonic and adult tissues. SPRY1 is considered a negative regulator of cellular signaling. In particular, it appears to both inhibit MAP kinase signaling following RTK activation, and block TCR signaling following antigen activation. It interacts with a number of molecules, including PLC-gama-1, LAT, CBL, caveolin-1 and SPRY2. Human SPRY1 is 319 aa in length and contains one CBL-TKB binding site (aa51-57) that is phosphorylated at Tyr53, a Ser-rich region (aa112-131), and a Cys-rich domain (aa181-306) that mediates intracellular translocation. In addition, SPRY1 undergoes serine phosphorylation, ubiquitination and palmitoylation, the latter which induces SPRY1 to associate with cell membranes. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% NaN3. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- E.coli-derived recombinant human SPRY1 (aa1-178; Accession # O43609).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human SPRY1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
