Splicing Factor 2 (SF2, ASF) (MaxLight 550)
Product Sizes
100ul
£1144.00
S5555-50-ML550-100UL
About this Product
- SKU:
- S5555-50-ML550
- Application:
- Immunoprecipitation, Western Blot, Immunofluorescence
- translate.label.attr.clone:
- 3G268
- Clonality:
- Monoclonal
- Extra Details:
- Numerous protein and ribonucleoprotein components are required to catalyze pre-mRNA splicing. The SR proteins are essential splicing factors. They regulate alternative splicing of many pre-mRNAs. Alternative splicing is a major mechanism for controlling expression of cellular and viral genes. SR proteins are a family of highly conserved serine/arginine rich RNA-binding proteins. Nine human SR proteins including SF2/ASF are currently known. Based on primary sequence and on motifs shared with other proteins, human SF2/ASF is a 248aa protein composed of four domains. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in a majority of cells. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Molecular Weight: Calculated MW: 27.7kD Western Blot: ~33kD (Doublet or triplet bands sometimes evident due to phosphorylation differences). Recommended Control: HeLa cells Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant human SF2/ASF protein.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the N-terminal portion (aa1-97) of human SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. Species Crossreactivity: mouse and rat. Crossreactivity expected with other species because SF2/ASF is highly conserved.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
