Splicing Factor 2 (SF2, ASF) (Biotin)
Product Sizes
100ul
£1144.00
S5555-50-BIOTIN-100UL
About this Product
- SKU:
- S5555-50-BIOTIN
- Application:
- Immunoprecipitation, Western Blot, Immunofluorescence
- translate.label.attr.clone:
- 3G268
- Clonality:
- Monoclonal
- Extra Details:
- Numerous protein and ribonucleoprotein components are required to catalyze pre-mRNA splicing. The SR proteins are essential splicing factors. They regulate alternative splicing of many pre-mRNAs. Alternative splicing is a major mechanism for controlling expression of cellular and viral genes. SR proteins are a family of highly conserved serine/arginine rich RNA-binding proteins. Nine human SR proteins including SF2/ASF are currently known. Based on primary sequence and on motifs shared with other proteins, human SF2/ASF is a 248aa protein composed of four domains. SF2/ASF exists predominantly or exclusively in a highly phosphorylated state in a majority of cells. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Molecular Weight: Calculated MW: 27.7kD Western Blot: ~33kD (Doublet or triplet bands sometimes evident due to phosphorylation differences). Recommended Control: HeLa cells Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant human SF2/ASF protein.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Purity:
- Purified by Protein A affinity chromatography from ascites.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the N-terminal portion (aa1-97) of human SF2/ASF protein comprising the RNA-recognition motif (RRM1). Reactivity is not affected by phosphorylation state of SF2/ASF. Species Crossreactivity: mouse and rat. Crossreactivity expected with other species because SF2/ASF is highly conserved.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
