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Antibodies

SGLT-1 (Sodium Glucose Transporter 1) (Not for Export EU)

Product Sizes
100ul
£704.00
S1010-86A-100UL
About this Product
SKU:
S1010-86A
Clonality:
Polyclonal
Extra Details:
The kidneys play a major role in the regulation of glucose levels. Kidneys filter ~180g of glucose per day from the blood, and this is mostly reabsorbed back into the blood in the proximal tubules. Typically, glucose is first absorbed within epithelium by a specific transporter protein, sodium glucose co-transporters (SGLT), in the brush-border membrane and then it is transported out of the cell across the basolateral membranes bya facilitated sugar transporter (GLUTs). 3 members of SGLTs (SGLT1-3) have been cloned and characterized from various species. Individual members of this family have identical predicted secondary structures with up to 14 transmembrane domains. SGLT1-3 genes code for protein of ~659-672 residues (75kD). Both N and C termini are predicted to be extracellular. There is ~60-70% homology between SGLT1-3. SGLTs transport a-methyl-D-glucoside (a-MDG), a non metabolized model substrate, in Na-dependent manner. SGLT1 does not discriminate a-MDG, glucose, and galactose. SGLT2/3 do not transport D-galactose efficiently. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000, ~70-77kD. ELISA: 1:10,000-1:50,000. Coat ELISA plates with control peptide (S1010-86F) at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Rabbit
Immunogen:
Synthetic peptide corresponding to 19aa near the extracellular domain 4 of mouse/rabbit SGLT-1, conjugated to KLH.
Isotype:
IgG
Physical State:
Supplied as a liquid in 0.05% sodium azide
Purity:
Serum
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse SGLT-1. Species Crossreactivity: rabbit, rat, human, porine, feline, canine, reptiles and birds. Species Sequence Homology: rabbit and sheep; 100%, equine and rat; 94%, porcine; 89%, chicken; 86%, human; 84%. mouse SGLT-4a/4b; 84%, human SAAT/SGLT-3; 78%, human and rat SGLT-2; 63%
Source:
mouse
Storage Conditions:
-20[o]C
Type:
Antibodies:Polyclonal Antibody