Semaphorin 4D (BB18, A8, GR3, CD100 antigen, SEMA4D, CD100, SEMAJ)
Product Sizes
100ug
£812.00
S0668-55H-100UG
About this Product
- SKU:
- S0668-55H
- Application:
- Western Blot
- translate.label.attr.clone:
- 9j16
- Clonality:
- Monoclonal
- Extra Details:
- Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150kD type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861aa Semaphorin 4D contains a 710aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120kD form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds Plexin B1, but also CD72 on B and dendritic cells. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is proangiogenic when expressed by tumor-associated macrophages. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml with lysates of mouse brain and thymus tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Phe24-Thr657 from mouse Semaphorin 4D, expressed in NS0 cells.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Semaphorin 4D. Does not crossreact with recombinant mouse Semaphorin 3F.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
