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Antibodies

SCF (Stem Cell Factor, CD117 c-kit, Steel Factor, Mast Cell Growth Factor, MGF) (Biotin)

Product Sizes
25ug
£479.00
S0400A-25UG
About this Product
SKU:
S0400A
Application:
Western Blot
Clonality:
Polyclonal
Extra Details:
Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA (Sandwich): 0.25-1.0ug/ml (100ul/well). The detection limit is 0.2-0.4ng/well for recombinant human SCF when used with compatible secondary reagents, ELISA (Direct): 1ug/ml (100ul/well). The detection limit is 0.2-04ng/well of recombinant human SCF when used with compatible secondary reagents, Western Blot: 0.1-0.2ug/ml detects human SCF. Used in conjunction with compatible secondary reagents the detection limit for recombinant human SCF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilution determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to human SCF, expressed in E. coli.
Isotype:
IgG
Physical State:
Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA to 0.1-1mg/ml. Do not vortex..
Purity:
Purified by immunoaffinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human SCF.
Source:
human
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Polyclonal Antibody