Scavenger Receptor BII (SR-BII) (Not for Export EU)
Product Sizes
100ul
£633.00
S0099-010-100UL
About this Product
- SKU:
- S0099-010
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- Clonality:
- Polyclonal
- Extra Details:
- High density lipoproteins (HDLs) play a critical role in cholesterol metabolism. HDL plasma concentrations are inversely correlated with risk for atherosclerosis. SR-BI and SR-BII (previously known as SR-BI.2) are the alternatively spliced products of a single gene. SR-BII differs from SR-BI in that the encoded c-terminal cytoplasmic domain is almost completely different. SR-BII binds HDLs mediating selective uptake of HDL cholesteryl ester; with an approximately 4-fold lower efficiency than SR-BI. Nuclease protection assays show SR-BII to be abundant in mouse tissues expressing SR-BI. SR-BII expression found in liver, adrenal glands, and testes. The role of SR-BII is not completely clear. Research suggests that it may be a functional HDL receptor. SR-BII mRNA results from the alternative splicing of SR-BI precursor transcripts with the SR-BII isoform mediating selective transfer of lipid between HDL and cells. The relative expression and functional activities of these two isoforms create a potential means of regulating selective lipid transfer between HDL and cells (1-6). Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Western Blot and Immunofluorescence. Knockdown Validated. Other applications not tested Recommended Dilution: Western Blot: 1:1000. A band of ~82kD is seen in tissues that express SR-BII such as liver, tested and adrenal glands. Immunofluorescence (IC): 1:25-1:100 Immunohistochemistry (paraffin): 1:25 Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to residues from mouse SR-BII (between residues 400-478) plus an N-terminal cysteine coupled to KLH.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid, 0.09% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse SR-BII. Species crossreactivity: mink, rat, bovine and hamster.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
