S100 Protein, beta, beta (FITC)
Product Sizes
100ul
£1221.00
S0052-14-FITC-100UL
About this Product
- SKU:
- S0052-14-FITC
- Application:
- Western Blot, FLISA
- translate.label.attr.clone:
- 1.B.660
- Clonality:
- Monoclonal
- Extra Details:
- S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100A is composed of an alpha and beta chain whereas S100B is composed of two beta chains. Antibody to S100 stains Schwannomas, ependymomas, astrogliomas, almost all benign and malignant melanomas and their metastases. S100 protein is also expressed in the antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in the paracortex of lymph nodes. S100 is a nervous tissue specific soluble protein made in glial cells. Present in sweat glands chongrocytes and myoepithelial cells. May be found as cytoplasmic, membranous or mixed throughout the areas of tumor. Usually cytoplasmic when present in normal tissues. Applications: Suitable for use in Western Blot, FLISA and Immunoaffinity Purification. Other applications not tested Recommended Dilutions: Optimal dilutions to be determined by researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Purified S100 protein from human brain.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Fluorescein isothiocyanate (FITC).
- Purity:
- Purified by Protein A affinity chromatography (≥ 90%). Purity tested by electrophoresis.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes S100 (beta, beta) and S100 (alpha, beta) protein.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
