Rhodamine (TRITC, Tetramethylrhodamine Isothiocyanate Isomer R) (HRP)
Product Sizes
200ug
£704.00
R1998-12H-200UG
About this Product
- SKU:
- R1998-12H
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- NI 308
- Clonality:
- Monoclonal
- Extra Details:
- The reactivity of the antiserum is directed to the TRITC molecule as tested in direct binding ELISA and immunoblotting. This antibody is intended to enhance the specific signal obtained with a monoclonal antibody or a polyclonal second antibody conjugated to TRITC. The sensitivity of the TRITC hapten-anti-hapten system makes it a valuable alternative to the biotin-avidin system. This finds particular application in a situation where there is weak monoclonal antibody binding to PBMCs expressing surface markers. This can also be applied to solid phase systems when used for the identification and/or quantitative determination of minute amounts of soluble specific antigens or antibodies. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry, Immunohistochemistry and Dot Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:100 Western Blot: 1:200 Immunohistochemistry (Paraffin): 1:10-1:40 Optimal dilutions to be determined by the researcher. Storage and Stability: Store lyophilized product at 4°C if to be used immediately within two weeks; store at -20°C for long-term storage. Upon reconstitution, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Highly purified Tetramethylrhodamine Isothiocyanate Isomer R (TRITC).
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP). Reconstitute with 500ul sterile ddH2O. Dilute further in PBS, pH 7.2.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes TRITC.
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
