Retinal Pigment Epithelium Specific Protein (Retinal Pigment Epithelium-specific 65kD Protein, RPE65, All-trans-retinyl-palmitate Hydrolase, LCA2, mRPE65, rd12, Retinol Isomerase, Retinoid Isomerohydrolase, RP20, sRPE65)
Product Sizes
100ul
£576.00
R1659-70A-100UL
About this Product
- SKU:
- R1659-70A
- Application:
- Flow Cytometry, Immunohistochemistry, Immunofluorescence, Western Blot
- translate.label.attr.clone:
- 8B99 (401.8B11.3D9)
- Clonality:
- Monoclonal
- Extra Details:
- RPE65 is a major protein of the retinal pigment epithelium (RPE). RPE65 is essential for the regeneration of rhodopsin in the visual cycle. Mutations in RPE65 are responsible for certain forms of autosomal recessive severe retinal dystrophy, including Leber Congenital Amaurosis (LCA). Loss of function results in a block in retinoid processing required for the synthesis of 11-cis retinal and the accumulation of retinyl esters in the RPE. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Western Blot: 1-2ug/ml, recognizes a band at ~65kD representing bovine RPE65 protein. Also been tested on recombinant human protein in COS7 cells. Immunohistochemistry (Frozen): 1:250 on murine retinal sections Immunohistochemistry (Paraffin): 1:250-1:500 Immunofluorescence (IC): 1:50-1:200 on ARPE19 cells Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Bovine RPE microsomal membrane proteins. Cellular Localization: Cytoplasmic, microsomeal and cell membrane. Species Sequence Homology: birds; 89%, zebrafish; 74%, sea lamprey; 71%.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes bovine RPE65. Species Crossreactivity: porcine, human, mouse, rat, canine, chicken, primate and Xenopus.
- Source:
- bovine
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
