Relaxin 3 (H3 Relaxin, Insulin-Like Peptide-7, INSL7)
Product Sizes
500ug
£1004.00
R1425-20B-500UG
About this Product
- SKU:
- R1425-20B
- Application:
- Western Blot
- translate.label.attr.clone:
- 8H147
- Clonality:
- Monoclonal
- Extra Details:
- Human Relaxin 3 is one of seven relaxin-like peptides belonging to the insulin superfamily (1-4). Unlike human relaxins 1 and 2, it does not play a role in reproduction but appears to be a neuropeptide involved in stress response in the brain stem (3-5). The single-chain human Prorelaxin 3 shares 83% and 80% amino acid (aa) sequence identity with mouse and rat prorelaxin 3, respectively.The 142 aa Relaxin 3 pre-proprotein is processed to remove a 25aa signal peptide and a connecting peptide (aa53-118). The resulting mature Relaxin 3 is a 5.5kD, 51aa secreted heterodimer of A (aa119-142) and B (aa26-52) peptides connected by two intermolecular disulfide bonds (1). Mature human Relaxin-3 is 96%, 94%, and 92% aa identical to porcine, canine, and mouse Relaxin 3, respectively. This is much higher identity between species than that seen for other relaxins. Relaxin 3 is thus suggested to be the ancestral relaxin family member (2). Relaxin 3 is the only known ligand for the G-protein-coupled receptor GPCR135, designated RXFP3 (4, 6). In rodents, GPCR135 is expressed primarily in the supraoptic and paraventricular nucleus (6). This region has connections to the dorsal tegmental region of the pons (also called the nucleus incertus), where expression of Relaxin 3 is highest (5). Relaxin 3 also binds the more widely-expressed LGR7 (RXFP1) receptor, but with lower affinity than that of Relaxin 2 (1, 7). Although binding of Relaxin 3 to LGR7 increases intracellular cAMP, binding to GPCR135 inhibits cAMP accumulation, indicating coupling to Gi, Go or Gz by this receptor (1, 5). Relaxin 3 expression does not overlap well with its other receptor, GPCR142, which instead appears to be the primary receptor for INSL5 (3, 8). Applications: Suitable for use in Direct ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml detected Relaxin 3 in recombinant human Relaxin 3 under non-reducing conditions only. Neutralization: The ND50 is ~1-5ug/ml in the presence of 25ng/ml recombinant human Relaxin 3. Measured by its ability to neutralize Relaxin 3-induced cAMP production in the THP-1 human acute monocytic leukemia cell line (9). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg26-Cys142 from human Relaxin 3 expressed in E. coli.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Relaxin 3. In direct ELISAs and Western Blots, reacts with the pro and mature forms of human Relaxin 3. Does not crossreact with the pro forms of recombinant human Relaxin 1 or Relaxin 2.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
