RANTES (Regulated on Activation, Normal T-cell Expressed and Secreted) (MaxLight 550)
Product Sizes
100ul
£672.00
R1100-11-ML550-100UL
About this Product
- SKU:
- R1100-11-ML550
- Application:
- Western Blot, Immunohistochemistry, FLISA
- translate.label.attr.clone:
- 8.PP.11
- Clonality:
- Monoclonal
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. RANTES (regulated upon activation, normal T-cell expressed and secreted) is a 70-amino acid CC chemokine originally cloned from T cell-specific sequences-enriched cDNA libraries. Both human and murine RANTES are chemotactic for monocytes. Human RANTES is also a chemoattractant for T cells and eosinophils, as well as an activator of basophils for histamine release and of eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. In contrast to other CC chemokines, RANTES is only slowly expressed after activation of leukocytes. It is involved in a number of pathological conditions like chronic inflammation and autoimmune diseases. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency (SIV). Applications: Suitable for use in Western Blot, Immunohistochemistry and FLISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Formalin fixed and paraffin embedded tissues. Requires HIER with citrate buffer, pH 6.0 FLISA: Sandwich Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human RANTES, expressed in E. coli.
- Isotype:
- IgG2b,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Purity:
- Purified by Protein A affinity chromatography from ascites
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human RANTES.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
