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Antibodies

RANKL (OPGL, ODF, Trance, Receptor Activator of NFkB Ligand, TNF-related Activation-induced Cytokine, CD254, RANK L,, TNFSF11)

Product Sizes
100ug
£639.00
R1075-12A-100UG
About this Product
SKU:
R1075-12A
Application:
Flow Cytometry, Immunohistochemistry, Immunofluorescence, Western Blot
translate.label.attr.clone:
6D724 (12A380)
Clonality:
Monoclonal
Extra Details:
TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells. RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival. TRANCE/RANKL is also important in T- and B-cell maturation. As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis. OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunocytochemistry, Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 4-6ug/ml Flow Cytometry: 1:10-1:1000 Immunohistochemistry (frozen): 1:10-1:500 Immunohistochemistry (paraffin): 1:50 - 1:250 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Mouse
Immunogen:
Bacterially expressed fusion protein containing amino acid residues 1-317 of mouse TRANCE.
Isotype:
IgG1,k
Physical State:
Supplied as a liquid in PBS, 0.05% sodium azide.
Purity:
Purified by Protein G affinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse RANKL. Species Crossreactivity: human
Source:
mouse
Storage Conditions:
-20[o]C
Type:
Antibodies:Monoclonal Antibody