Protein serine/threonine phosphatase 2C alpha/beta
Product Sizes
100ug
£736.00
P9108-16-100UG
About this Product
- SKU:
- P9108-16
- Application:
- Immunoprecipitation, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 5-10ug/ml Immunoprecipitation: 5-10ug/ml Optimal dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to NH2-Arg-Tyr-Gly-Leu-Ser-Ser-Met-Gly-Trp-Arg-Val-Glu-COOH, conjugated to KLH
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.08% sodium azide.
- Purity:
- Purified by ammonium sulfate precipitation
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Protein serine/threonine phosphatase 2C alpha/beta (44-46kD). Species Crossreactivity: human, mouse, rat and bovine. Does not crossreact with other protein phosphatase isoforms.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
