Protein Serine/Threonine phosphatase 2A/B alpha
Product Sizes
100ug
£736.00
P9108-14-100UG
About this Product
- SKU:
- P9108-14
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Type 2 protein phosphatases are serine/threonine phosphatases which can preferentially dephosphorylate the alpha subunit of phosphorylase kinase and are insensitive to inhibitor 2 treatment. Protein phosphatase 2A (PP2A) can be distinguished from PP2B and PP2C which require Ca2+ and Mg2+, respectively, by its partial activity in the absence of divalent cations. PP2A activity is potently inhibited by treatment with okadaic acid and shows much stronger activity toward histone phosphorylated by Protein Kinase C and casein phosphorylated by Protein Kinase A than other protein phosphatases. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 5-10ug/ml Immunocytochemistry: 5-10ug/ml Optimum dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa14-27, NH2-Phe-Ser-Gln-Val- Lys-Gly-Ala-Val-Asp-Asp-Asp-Val-Ala-Glu-COOH, conjugated to KLH.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.08% sodium azide.
- Purity:
- Purified by ammonium sulfate fractionation.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes Protein Serine/Threonine phosphatase 2A/B alpha (55kD). Does not crossreact with other protein phosphatase isoforms. Species Crossreactivity: human, mouse, rat and bovine.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
