PPP1R14A, Recombinant, Human (Protein phosphatase 1 regulatory subunit 14A, CPI17, CPI-17, PPP1INL)
Product Sizes
100ug
£576.00
P9107-30Z-100UG
About this Product
- SKU:
- P9107-30Z
- Extra Details:
- PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization. Recombinant protein corresponding to aa1-167 of human PPP1R14A fused to a His-tag at the N-terminal expressed in E. coli (NP_150281). Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 18
- Physical State:
- Supplied as a liquid in 20mM Tris pH 8.0, 2mM EDTA, 1mM DTT, 10% glycerol.
- Purity:
- ≥ 90% by SDS-PAGE. Purified using conventional chromatography techniques.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
