Protein Kinase C (PKC)
Product Sizes
100ug
£633.00
P9103-16B2-100UG
About this Product
- SKU:
- P9103-16B2
- Application:
- Western Blot, Immunofluorescence, Radioimmunoassay, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
- translate.label.attr.clone:
- 3C174
- Clonality:
- Monoclonal
- Extra Details:
- Protein Kinases C (PKC) are a family of serine/threonine protein kinases that play key roles in extracellular signal transduction. MC5 blocks partial proteolysis of PKC by trypsin in vitro. Inappropriate activation of PKC has been implicated in the pathophysiology of many diseases, including diabetes mellitus. The PKC family now includes 11 isoforms that are differentially expressed in various cell types. Applications: Suitable for use in RIA, Flow Cytometry, Immunohistochemistry, Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10-1:1000. Immunohistochemistry (Frozen/paraffin): 1:200-1:500. Immunofluorescence (IC): 1:100-1:500. ELISA: 1:100-1:2000. Western Blot: 1:1000. Immunoprecipitation: 1:10-1:100. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Purified bovine brain PKC.
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in Tris-Glycine, 0.15M sodium chloride, 0.05% sodium azide
- Purity:
- Purified by Protein A affinity chromatography
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes bovine alpha, beta and gamma PKC isoforms. Epitope mapped to the hinge region of PKC, residues 293-317. Species Crossreactivity: mouse, rat, porcine, human, canine, fish, rabbit and sheep
- Source:
- bovine
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
