PLTP (Plasma Phospholipid Transfer Protein, Lipid Transfer Protein II) (Biotin)
Product Sizes
50ug
£889.00
P4278-02A-50UG
About this Product
- SKU:
- P4278-02A
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- PLTP (phospholipid transfer protein) is a 65-81kD, secreted glycoprotein member of the BPI/LBP family of proteins. It is expressed by multiple cell types, circulates bound to HDL, and mediates the transfer of phospholipids and cholesterol from apoB-containing lipoproteins to HDL. Mature human PLTP is 476 aa in length. It contains an N-terminal lipid transfer domain and a C-terminal HDL-binding domain. There is one essential intrachain disulfide bond (Cys146-Cys185). There are four potential PLTP splice variants. Two show alternate start sites at Met21 and Met89. Two others demonstrate aa substitutions; one shows a one aa (Lys) substitution for aa110-162, and a second shows a four aa substitution for the N-terminal 35 aa. Over aa18-493, human PLTP shares 92% and 83% aa identity with porcine and mouse PLTP, respectively. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1ug/ml using recombinant human PLTP Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Mouse myeloma cell line NS0-derived recombinant protein corresponding to Glu18-Val493 (Glu18Val) from human PLTP isoform 1.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human PLTP. ~15% cross-reactivity with recombinant mouse PLTP
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
