PF4 (Platelet Factor 4) (PE)
Product Sizes
100Tests
£806.00
P3360-01E-100TESTS
About this Product
- SKU:
- P3360-01E
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- 7H2
- Clonality:
- Monoclonal
- Extra Details:
- Platelet Factor 4 (PF4), is a member of the CXC chemokine family. Human PF4 is a 101 amino acid (aa) protein with a 32aa signal sequence and a 70aa mature protein. PF4 has homology with IL-8 and beta -thromboglobulin. Human and mouse PF4 share a 64% identity. PF4 contains several heparin-binding sites at the C-terminal region. The active protein consists of a tetramer composed of individual PF4 subunits. Megakaryocytes synthesize PF4 and store it as tetramers in alpha-granules. The PF4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, PF4 lacks chemotactic activity for polymorphonuclear granulocytes. PF4 does not contain an ELR motif. However, many other functions have been observed for PF4. PF4 is involved in monocyte survival and differentiation into macrophages, and it has anti-angiogenic activity. PF4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors, contributing to its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as PF4 binding sites; affinity is controlled by the degree of sulfation of these chains. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 5x10e6 cells using human dendritic cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Glu32-Ser101 of human PF4 expressed in E. coli.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human PF4. Does not cross-react with recombinant mouse CXCL6, recombinant human CXCL6, recombinant human CXCL7, recombinant mouse CXCL13 or recombinant human CCL21.
- Source:
- human
- Storage Conditions:
- 4[o]C Do not freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
