PEA-15 (phosphoprotein enriched in astrocytes 15 Astrocytic phosphoprotein PEA-15, HMAT1, HUMMAT1H, MAT1, MAT1H, PED, Phosphoprotein enriched in diabetes)
Product Sizes
100ug
£876.00
P3139-86-100UG
About this Product
- SKU:
- P3139-86
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- PEA-15 (Phosphoprotein enriched in astrocytes of 15kD) is a 15kD cytoplasmic protein that regulates cell proliferation and apoptosis. It is expressed in multiple cell types such as astrocytes; skeletal muscle and adipocytes. Human PEA-15 is 130aa in length. It contains a death effector domain (aa3-81), plus two regulatory Ser phosphorylation sites. In a quiescent cell, it is either non-phosphorylated or constitutively phosphorylated at Ser116. Non-phosphorylated PEA-15 binds ERK, inhibiting cell proliferation. Phosphorylation at Ser116 promotes PEA-15 binding to FADD, blocking apoptosis. And activation-induced phosphorylation at Ser104 blocks PEA-15 binding to ERK, promoting cell proliferation. There is one potential alternate start site 21aa upstream of the standard start site, and a second isoform that shows an Asn substitution for aa 36-58. Full-length human and mouse PEA-15 show 99% aa identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1.0ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, PBS.. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant human PEA-15 aa 2-130; Q15121 (E. Coli).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human and mouse PEA-15.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
