PCNA (Proliferating Cell Nuclear Antigen)
Product Sizes
100ug
£685.00
P3115-11B-100UG
About this Product
- SKU:
- P3115-11B
- Application:
- Immunoprecipitation, Flow Cytometry, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 3C169
- Clonality:
- Monoclonal
- Extra Details:
- Proliferating cell nuclear antigen (PCNA) is a 36kD nuclear protein associated with the cell cycle, a nuclear protein vital for cellular DNA synthesis. Proliferating cell nuclear antigen was originally identified by immunofluorescence as a nuclear protein whose appearance correlated with the proliferate state of the cell. PCNA is required for replication of DNA in vitro and has been identified as the auxiliary protein (cofactor) for DNA polymerase delta. The anti-PCNA antibodies react with the nuclei of proliferating cells. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:5000. Detects a band at 30kD. Immunohistochemistry (Paraffin Embedded): 10ug/ml. Heat-mediated citrate buffer antigen retrieval. Optimal dilutions to be determined by the researcher Postive Control: DT40 B lymphocyte whole cell lysate (chicken) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- PCNA fusion obtained from pC2T
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat PCNA p36 protein expressed at high levels in proliferating cells. Species Crossreactivity: human, mouse, yeast (S. pombe and S. cerevisiae), chicken and Drosophila melanogaster.
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
