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Antibodies

Pax6 (Paired Box Gene 6, AN2, D11S812E, MGDA, WAGRy)

Product Sizes
100ug
£659.00
P3113-84B1-100UG
About this Product
SKU:
P3113-84B1
Application:
Western Blot
translate.label.attr.clone:
8C259
Clonality:
Monoclonal
Extra Details:
Retinal progenitor cells (RPCs) retain the ability to generate different retinal cell types. RPCs appear to be retained in a progenitor state by the action of Notch-Delta signaling and downstream effectors of Notch, like the bHLH transcription factor Hes1. The most uncommitted RPCs (retinal stem cells) in the ciliary margin of the Xenopus retina were shown to coexpress the transcription factors Pax6, RX1, and Six3 prior to the activation of neurogenic genes (Notch, Delta, bHLH) and the subsequent steps toward retinal neurogenesis. Pax6 is required for the multipotent state of retinal progenitor C. Upon Pax 6 inactivation, the potential of RPCs becomes entirely restricted to only one of the cell fates normally available to RPCs resulting in the exclusive generation of amacrine interneurons. Pax6 has been found to directly control the transcriptional activation of retinogenic bHLH factors that bias subsets of RPCs towards different retinal cell fates, thereby mediating the full retinogenic potential of RPCs. Applications: Suitable for use in ELISA, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Mouse
Immunogen:
Partial recombinant protein corresponding to human Pax6
Isotype:
IgG1
Physical State:
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
Purity:
Purified by Protein G affinity chromatography from hybridoma culture supernatant
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human Pax6.
Source:
human
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody