p45 (SKP2)
Product Sizes
100ug
£850.00
P1001-23B-100UG
About this Product
- SKU:
- P1001-23B
- Application:
- Immunoprecipitation, Western Blot, Immunofluorescence
- translate.label.attr.clone:
- 3G225
- Clonality:
- Monoclonal
- Extra Details:
- The F-box protein p45SKP2 is the substrate-targeting subunit of the protein ligase SCFSKP2 and is frequently overexpressed in transformed cells. Expression of p45SKP2 in quiescent fibroblasts promotes p27Kip1 degradation, allows the generation of cyclin-A-dependent kinase activity, and allows entry into the S phase of the cell cycle. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence/Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: ELISA: 0.1-1ug/ml Western Blot: 1:250-1:500 Immunoprecipitation: 5ug Immunofluorescence (IC): 1:250 Optimal dilutions to be determined by the researcher. Positive Control: HeLa, Jurkat and 293T cell lines. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Full-length recombinant protein corresponding to human p45 (SKP2).
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human p45 (SKP2).
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
