p27Kip1, phosphorylated (Thr198)
Product Sizes
100ug
£812.00
P1001-11D-100UG
About this Product
- SKU:
- P1001-11D
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- The cyclin-dependent kinase (cdk) inhibitor p27 Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27 Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27 Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4-imidazolecarboxamide riboside) or glucose withdrawal. Applications: Western Blot: 0.5ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Phospho-peptide containing human p27 Kip1 (T198) site
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human phospho-p27 Kip1 (T198).
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
