Ogg1 (8-Oxoguanine DNA Glycosylase, DNA Lyase, DNA-(Apurinic or Apyrimidinic Site) Lyase, HMMH, HOGG1, MUTM, N-glycosylase, OGH1) (Biotin)
Product Sizes
100ul
£780.00
O5700-01B-100UL
About this Product
- SKU:
- O5700-01B
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- 8-hydroxyguanine, a form of oxidative DNA damage induced by free radicals, causes G:C to T:A transversion. In E. coli, three DNA repair enzymes exist to prevent the mutagenic effects of 8-hydroxyguanine. One of these enzymes, MutM, was found to have a functional yeast (yOgg1) and human (hOgg1) homologue. hOgg1 proteins efficiently release the 8-hydroxyguanine opposite the pyrimidine from DNA and cleave the AP site in a manner similar to bacterial and yeast enzymes. Genetic backgrounds in control of the repair of damaged DNA are involved in the susceptibility to cancer development. The hOgg1 gene has been mapped to region 3p26.2, a region showing loss of heterozygosity (LOH) in a variety of cancers. In particular, 3p25-p26 is a common LOH region in lung cancer. Recent work has demonstrated that Ogg plays an important role in CAG expansion, a characteristic of several neurodegenerative diseases. Ogg appears to be responsible for progressive expansion of poly-Q tracts in response to oxidative damage. Thus, Ogg provides a direct link between DNA damage and toxicity in neurons. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:1000 Immunohistochemistry: 1:25-1:100 Optimal dilutions to be determined by researcher. Positive Control: Jurkat whole cell extracts Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Peptide corresponding to aa1-100 from human Ogg1. Species Sequence Homology: rabbit and porcine
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Ogg1. Species Crossreactivity: primate and rat
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
