Organic Anion Transporter 1 (OAT1) (Not for Export EU)
Product Sizes
100ul
£704.00
O3003-01-100UL
About this Product
- SKU:
- O3003-01
- Clonality:
- Polyclonal
- Extra Details:
- Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called "renal secretion". Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides; oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Rat Kidney PAH transporter, termed OAT1 or ROAT1, encodes a protein of 551 aa. Human OAT1 (hOAT1) is 563 aa and its alternatively spliced isoform hOAT2 is 550 aa (missing 13 aa from 523-533 aa). OAT1 has wide substrate selectivity, covering endogenous substrates such as cyclic nucleotides, prostaglandin and uric acid, and a variety of drugs (e.g., antibiotics, non-steroidal anti-inflammatory drugs, diuretics, anti-neoplastic drug, and a uricosuric drug). OAT1 has been localized on the basolateral membrane of the proximal tubule in the kidney. Weak expression was also detected in brain. OAT1 has ~95 identity with mouse NKT, an ortholog of rat OAT1. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 using ECL technique. ELISA: 1:10,000-50,000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- A synthetic peptide corrsponding to 16aa near the cytoplasmic, C-terminus of rat OAT1, conjugated to KLH. Species Sequence Homology: mous and, rabbit; 87%, human; 81%.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid, 0.05% sodium azide
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Organic Anion Transporter 1. No significant sequence homology is detected with other OATs or other proteins
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
