Novel Organic Cation Transporter 1 (OctN1) (Not for Export EU)
Product Sizes
100ul
£704.00
N5376-52-100UL
About this Product
- SKU:
- N5376-52
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic cations from the blood by an active process called "renal secretion". Functional studies have identified two distinct categories of organic cation transporters (Octs): a system driven by transmembrane potential difference that governs the influx of cations, whereas the H+-gradient-dependent transport system may mediate the efflux of organic cations. Several multispecific, potential-sensitive transporters (Oct1-3) and H+-dependent transporters (OctN1-3) have been cloned and characterized from various tissues. Oct superfamily of proteins shares high degree of sequence homology, display up to 12 transmembrane domains with cytoplasmic N and C-terminus. A novel transporter termed OctN1, has been cloned and characterized human fetal liver, which carries a nucleotide birding site motif. OctN1 (human 551aa, mouse 55aa) shares ~32% homology with Oct1-3. It is strongly expressed in adult kidney, trachea, bone marrow, and fetal liver, and several tumor cells, but not in adult human liver. OctN1 mediated uptake of TEA in pH dependent manner. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml Optimal dilutions to be determined by the researcher. Control Peptide: N5376-50: Novel Organic Cation Transporter 1, Mouse, Control Peptide (OctN1) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 20aa from mouse OctN1 at C-terminal, conjugated to KLH. Species Sequence Homology: rat, 88%; human, 61%
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Novel Organic Cation Transporter 1. No significant sequence homology is detected with other Octs or OctNs.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
