Notch1 (Notch Homolog Protein 1, hN1, Translocation-associated Notch Protein TAN-1) (Biotin)
Product Sizes
50ug
£934.00
N5375-03-50UG
About this Product
- SKU:
- N5375-03
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Notch-1 (so named for “notches” in fly wings; also TAN-1) is a 300kD member of the Notch family of glycoproteins. It is associated with gene activation in both embryo and adult. Human Notch-1 is a 2538 amino acid (aa) type I transmembrane glycoprotein. It undergoes Golgi processing to generate a heterodimer composed of a 180-200kD disulfide-linked extracellular domain (aa 18-1664) and a 120kD membrane-bound segment (aa 1665-2556). Upon ligand binding, the 110kD segment undergoes two cleavages which generate an NICD (notch intrImmunogen. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.2ug/mL detection limit for rhNotch-1 ICD ~ 0.5ng/lane under non-reducing and reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- E. coli-derived, recombinant human Notch-1 (aa 1754 - 2556)
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, BSA. Labeled with Biotin. Reconstitute with 250ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Notch-1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
