NM23-H1 (H2) (Non-metastatic protein 23 homolog 1, NDKA)
Product Sizes
100ug
£869.00
N2900-65G-100UG
About this Product
- SKU:
- N2900-65G
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- NM23-H1 (Non-metastatic protein 23 homolog 1; also NDKA) is a 17-20kD member of the NDK family of enzymes. NM23-H1 is ubiquitous in expression, and performs multiple functions. It forms disulfide-linked homohexamers, and heterohexamers with NM23-H2, generating a nucleoside diphosphate kinase that catalyzes a phosphoryl transfer from ATP to a nucleoside diphosphate. It also shows histidine protein and Ser/Thr protein kinase activity, and forms covalent linkages with molecules diverse as p53 and STRAP. It is found both intracellularly, and in blood at ng/ml concentrations. Human NM23-H1 is 152 amino acids (aa) in length, contains one NDP kinase domain (aa 5-134) and shows acetylation at Ala2 and Lys56, plus phosphorylation at Tyr52, Thr94, Ser122 and Ser125. Human NM23-H1 shares 89% aa identity with human 17-18kD NM23-H2, and 94% aa identity with mouse NM23-H1. A second H1 isoform named NM23-H1B with 25 additional aa at the N-terminus has also been described. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- E. coli-derived recombinant protein corresponding to Met1-Glu152 from human NM23‑H1.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute in 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NM23-H1 (H2). Species Crossreactivity: mouse and rat.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
