NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA) (Biotin)
Product Sizes
100ul
£800.00
N2303-01B-100UL
About this Product
- SKU:
- N2303-01B
- Application:
- Flow Cytometry, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 4H211 (112A1021)
- Clonality:
- Monoclonal
- Extra Details:
- NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:2000 Flow Cytometry: 1ul/10e6 cells Immunohistochemistry (Paraffin): 1:10-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein. Species Sequence Homology: bovine, gorilla, New World monkey and porcine.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Biotin.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NFkB. NF-kB (nuclear factor kB). Species Crossreactivity: Mouse and rat
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
