NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA)
Product Sizes
100ug
£704.00
N2303-01A-100UG
About this Product
- SKU:
- N2303-01A
- Application:
- Flow Cytometry, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 4H211 (112A1021)
- Clonality:
- Monoclonal
- Extra Details:
- NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65. Applications: Suitable for use in Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 25ug/ml Flow Cytometry: 0.25-0.5ug/10e6 cells Immunohistochemistry (Frozen): 5-15ug/ml Immunohistochemistry (Paraffin): 5ug/ml Optimal dilutions to be determined by the researcher. Positive Control: NFkB p105/p50 Overexpression Lysate (Native) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NFkB p65. Species Crossreactivity: mouse and rat
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
