NFkB, p65 (NF-kB, Nuclear Factor kappa B, RelA) (HRP)
Product Sizes
100ul
£1099.00
N2302-01C-HRP-100UL
About this Product
- SKU:
- N2302-01C-HRP
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- Clonality:
- Polyclonal
- Extra Details:
- NF kappa B is a heterodimer consisting of a 50kD DNA binding subunit and a 65kD transactivating subunit (RelA). The p50/p65 heterodime is present in the cytoplasm in inactive form bound to its inhibitor, IkB. Upon stimulation of cells with stimuli like LPS, Interleukin-1, IkB gets phosphorylated and degraded by proteosomes releasing the activated NFkB which goes to the nucleus inducing gene expression. NFkB was originally identified as transcriptional activator for kappa light chain gene in B cells. Applications: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry (Formalin/paraffin): Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat cells, Placenta Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the C-terminus of human NF kappa B.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NF kappa B Species Crossreactivity: mouse, rat, hamster, rabbit, bovine, porcine, canine, Drosophila and Xenopus.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
