NFkB2 (Nuclear Factor kappa B)
Product Sizes
100ug
£863.00
N2301-39B-100UG
About this Product
- SKU:
- N2301-39B
- Application:
- Western Blot, ChIP
- translate.label.attr.clone:
- 7H260
- Clonality:
- Monoclonal
- Extra Details:
- Nuclear Factor kappa B2 (NFκB2 or NFκB p52) is a member of the NFκB/Rel family of transcription factors. NFκB2 dimerizes with other members of the NFκB/Rel family to regulate expression of genes that participate in immune, apoptotic, and oncogenic processes. Applications: Suitable for use in ChIP, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Chromatin Immunoprecipitation (ChIP): 5ug per 5x10e6 cells Immunocytochemistry: 3-25ug/ml using immersion fixed human peripheral blood mononuclear cells and HeLa human cervical epithelial carcinoma cell line Western Blot: 0.5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Asn447 of human NFkB2, expressed in E. coli.
- Isotype:
- IgG1
- Physical State:
- Supplied as a powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NFkB2. Does not detect mouse NFkB2 in NIH 3T3 cells.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
