Neurotensin Receptor 1 (NTR1) (Not for Export EU)
Product Sizes
100ul
£704.00
N2177-25-100UL
About this Product
- SKU:
- N2177-25
- Clonality:
- Polyclonal
- Extra Details:
- Neurotensin (NT) is an endogenous tridecapeptide neurotransmitter that influences distinct central and peripheral physiological functions in mammals. Central administration of NT modulates dopaminergic transmission and triggers hypothermic and naloxone-insensitive analgesic responses, whereas peripheral effects include hypotension, decrease gastric acid release, potentiation of lipid digestion. NT is widely distributed throughout the CNS. It has been localized to catecholamine-containing neurons. NT initiates its biological action by interacting with two distinct G-protein coupled receptors (NTR1 and NTR2). Recently, a third receptor NTR3 has been identified that is identical to gp95/sortilin and it is not coupled via the G-proteins. All three receptors bind NT through its C-terminal hexapeptide sequence (8 RRPYIL 13). Biologically active NT (NT8-13) has also been shown to interact with the extracellular domain 3 (between TM6-7) of NTR1. NTR1/NTRH/NTSR1/NT1 (mouse/rat 424 aa; human 418 aa, chromosome 20q1; ~84% interspecies sequence homology) is the levocabastine-insensitive, high affinity receptor for NT. It has the typical structure of GPCR: 7 TM domains with extracellular N-terminus and cytoplasmic C-terminus. It is shown to mediate a number of peripheral and central NT responses, including the neuroleptic-like effects of the peptide. It is highly expressed in the brain, small intestine, and other peripheral tissues. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000. NTR1 is ~52-55kD in rat brain and it may be differentially glycosylated. ELISA: 1:100,000 using 50-100ng N2177-20 control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- An 18aa peptide sequence within the mid-region, extracellular location of mouse NTR1. (KLH). Species sequence homology: 84% rat and human. No significant sequence homology exist with NTR2 or NTR3 or other GPCR.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium sodium azide, 40% glycerol.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Neurotensin Receptor 1 (NTR1).
- Source:
- mouse
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
