Neuron Specific Enolase, gamma (gNSE)
Product Sizes
1mg
£934.00
N2175-09-1MG
About this Product
- SKU:
- N2175-09
- Application:
- Immunohistochemistry
- translate.label.attr.clone:
- 1.B.652
- Clonality:
- Monoclonal
- Extra Details:
- Enolases are homo- or heterodimers of the three subunits: alpha, 46kD, beta, 44kD, and gamma, 46kD. The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:100-1:300 (Formalin-fixed and paraffin-embedded sections). Optimal dilutions to be determined by the researcher. Matched Pair for ELISA: Capture: Conjugate: N2175-09 N2175-06 N2175-06 N2175-09 Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Purified neuron-specific enolase from human brain.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human gamma enolase subunit. No detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in Immunoblot of human brain extracts.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
