Neuron Specific Enolase, gamma (gNSE)
Product Sizes
1mg
£934.00
N2175-07-1MG
About this Product
- SKU:
- N2175-07
- Application:
- Immunohistochemistry
- translate.label.attr.clone:
- 1.B.650
- Clonality:
- Monoclonal
- Extra Details:
- Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD) and gamma (46kD). The alpha-subunit is expressed in most tissues. The beta-subunit is expressed only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed and paraffifn-embedded sections. Optimal dilutions to be determined by the researcher. Matched Pairs: Capture: N2175-06, N2175-07 Detection: N2175-07, N2175-06 Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Purified neuron-specific enolase from human brain.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography from ascites (≥ 95%). Purity is tested by electrophoresis.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human gamma enolase. Specific for the gamma subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
