Neuron Specific Enolase, gamma (gNSE) (APC)
Product Sizes
100ul
£1082.00
N2175-07-APC-100UL
About this Product
- SKU:
- N2175-07-APC
- Application:
- Immunohistochemistry, FLISA
- translate.label.attr.clone:
- 1.B.650
- Clonality:
- Monoclonal
- Extra Details:
- Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD) and gamma (46kD). The alpha-subunit is expressed in most tissues. The beta-subunit is expressed only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. Applications: Suitable for use in FLISA and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: Formalin-fixed and paraffifn-embedded sections. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Allophycocyanin (APC) is light sensitive. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Purified neuron-specific enolase from human brain.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Purity:
- Purified by Protein G affinity chromatography (≥ 95%). Purity is tested by electrophoresis.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Gamma enolase. Specific for the gamma subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
