Neuron Specific Enolase, gamma (gNSE) (Biotin)
Product Sizes
100ul
£1075.00
N2175-06-BIOTIN-100UL
About this Product
- SKU:
- N2175-06-BIOTIN
- Application:
- Immunohistochemistry
- translate.label.attr.clone:
- 1.B.649
- Clonality:
- Monoclonal
- Extra Details:
- Enolases are homo- or heterodimers of the three subunits: alpha (46kD), beta (44kD) and gamma (46kD). The alpha-subunit is expressed in most tissues. The beta-subunit is expressed only in muscle. The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Purified neuron-specific enolase from human brain.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Biotin.
- Purity:
- Protein A Sepharose chromatography. Purity is tested by electrophoresis.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Gamma-enolase. Specific for the gamma subunit without detectable crossreactivity with the alpha or beta subunits. Reacts with 45kD band corresponding to NSE in immunoblotting of human brain extracts.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
