Neurofilament L, 68kD (NF-L) (MaxLight 550)
Product Sizes
100ul
£1106.00
N2160-01G-ML550-100UL
About this Product
- SKU:
- N2160-01G-ML550
- Application:
- Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, FLISA
- translate.label.attr.clone:
- 3G203
- Clonality:
- Monoclonal
- Extra Details:
- Neurofilaments are intermediate (10-12nm) filaments located specifically in neurons. There are three classes of neurofilaments: NF-L (68kD), NF-M (160kD). And NF-H (200kD). The neurofilaments are long helical proteins which polymerize to form a rigid cytoskeleton in the neuron. This polymerized network is composed of all three filaments, and the stoichiometry of association varies during development. Neurofilaments are posttranslationally modified both by phosphorylation and glycosylation. Like other intermediate filament proteins, how phosphorylation likely mediates neurofilament function remains unclear. Neurofilament proteins (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal axons throughout the central and peripheral nervous system. Since NFPs are major structural proteins and biochemically quite stable, antibodies to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original cell type of the tumor. In addition, the normal NFP staining pattern is altered in a variety of human diseases including Alzheimer’s disease, diffuse cortical Lewy body disease, Parkinson’s disease and amyotrophic lateral sclerosis (Lou Gehrig’ disease). Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry and Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Enzymatically dephosphorylated porcine neurofilament proteins
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with MaxLight™550.
- Purity:
- Purified from ascites fluid.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Reacts with the 68kD polypeptides of human neurofilament and higher vertebrae. It specifically recognizes a phosphate-independent epitope on NF-L.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
