Nogo-B (Foocen, KIAA0886, My043, Nbla00271, Nbla10545, Neurite Outgrowth Inhibitor, Neuroendocrine-specific Protein, NI220/250, NSP, NSP-CL, Reticulon-4, Reticulon-5, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, RTN-x, RTN-X, SP1507, SP1507)
Product Sizes
100ug
£748.00
N2116-41C-100UG
About this Product
- SKU:
- N2116-41C
- Application:
- Immunohistochemistry
- translate.label.attr.clone:
- 11n137
- Clonality:
- Monoclonal
- Extra Details:
- Nogo-B, is a 55kD splice form of Nogo-A/Reticulon-4 and is primarily expressed in vascular endothelial cells. Nogo-A contains a large N-terminal cytoplasmic domain and a reticulon region that consists of two transmembrane segments with an intervening noncytoplasmic domain. Nogo-B lacks most of the N-terminal domain but retains the reticulon domain. It inhibits neointimal hyperplasia in the vasculature and is downregulated in atherosclerotic tissue and thoracic arterial aneurisms. Interaction of Nogo-B with NgBR promotes endothelial cell proliferation and migration. Within aa1-183, mouse Nogo-B shares 76-94aa sequence identity with human and rat Nogo-B, respectively. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 8-25ug/ml using immersion fixed frozen sections of adult mouse heart Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Met1-Ile183 (predicted) from Nogo-B expressed in NSO cells.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Nogo-B. In direct ELISAs, less than 25% cross-reactivity with recombinant human (rh) Nogo-B and recombinant rat Nogo-A and no cross-reactivity with rhNogo-A is observed.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
