Nogo-B (Foocen, KIAA0886, My043, Nbla00271, Nbla10545, Neurite Outgrowth Inhibitor, Neuroendocrine-specific Protein, NI220/250, NSP, NSP-CL, Reticulon-4, Reticulon-5, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, RTN-x, RTN-X, SP1507, SP1507)
Product Sizes
100ug
£876.00
N2116-41A-100UG
About this Product
- SKU:
- N2116-41A
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Nogo-B (A No-Go for neurite outgrowth Isoform B; also reticulon-4 isoform 2 and RTN-xS) is a 49-51kD member of the reticulon protein family. It is widely expressed, being reported everywhere but liver. Nogo-B appears to be principally expressed in the ER, but does have a receptor (NgBR) on cell surfaces. Intracellularly, Nogo-B is known to interact with Bcl-xl and Bcl-2, and may play in role in both apoptosis and angiogenesis. Human Nogo-B is a two transmembrane, 373aa protein. It contains an N-terminal cytoplasmic domain (aa1-199), two transmembrane segments (aa200-219 and 315-335), a luminal region (aa220-314) and a C-terminal cytoplasmic domain (aa336-373). Nogo-B contains multiple phosphorylation sites and undergoes acetylation. Caspase-7 cleaves Nogo-B between Asp14Ser15, generating a 45-47kD fragment. Nogo-B is generated from a full-length 205kD Nogo-A by the deletion ofaa186-1004. Overaa1-200, human Nogo-B shares 87% aa identity with mouse Nogo-B1. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml ELISA: 0.5-1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% NaN3. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- CHO cell-derived, recombinant human Nogo-B (rhNogo-B; aa1-200; Accession #NP_722550).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Nogo-B. Species Crossreactivity: mouse.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
