Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes) (Biotin)
Product Sizes
50ug
£940.00
N2060-10B-50UG
About this Product
- SKU:
- N2060-10B
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Nestin is a 240kD class VI intermediate filament protein that was first identified in neuroepithelial stem cells. It has been used extensively as a marker to identify central nervous system (CNS) stem cell populations. In addition, Nestin has been found to be a marker for multi-lineage progenitor cells. Over the region used for immunization, rat Nestin shares 76% aa sequence homology with the mouse protein. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.1-0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile TBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Purified, E. coli-derived, recombinant rat Nestin (rrNestin; aa544-776).
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Nestin. Species sequence homology: mouse (76%).
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
