NHE1 (Na, H Exchanger 1) (Not for Export EU)
Product Sizes
100ul
£704.00
N2015-08-100UL
About this Product
- SKU:
- N2015-08
- Application:
- Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Na+/H+ exchangers (NHE) of mammalian cells are plasma membrane intrinsic proteins mediating exchange of N+ and H+ ions in various tissues. The NHE catalyzes the electroneural transport of extracellular Na+ for intracellular H+. They play a major role in regulation of intracellular pH (pHi) in addition to trans-cellular absorption of Na+, cell volume regulation and possibly in cell proliferation. These primary functions of the Na+/H+ exchanger have been related to many pathophysiological states, include hypertension, organ growth and hypertrophy, regression of cancer and renal intestinal disorders. At least 7 NHE isoforms (NHE1-7) have been cloned so far. They are all similar in their primary structure and predicted to have 10-12 transmembrane domains. The C-terminal domain of NHEs are predicted to be intracellular. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Control Peptide: N2015-05 Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- A 22aa peptide within the cytoplasmic, c-terminal domain of the rat NHE1, coupled to KLH.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS and 0.05% sodium azide, before the addition of glycerol to 40%
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the 22aa sequence of NHE1 in human, mouse, rat, bovine, pig and rabbit. 90% homology with hamster.
- Source:
- rat
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
