NEDD9 (Neural Precursor Cell Expressed Developmentally Down-regulated protein 9, Enhancer of Filamentation 1, hEF1, CRK-associated Substrate-related Protein, CAS-L, CasL, Cas Scaffolding Protein Family Member 2, NEDD-9, Renal Carcinoma Antigen NY-REN-12, p105, CASL, CASS2)
Product Sizes
100ug
£767.00
N0580-09A1-100UG
About this Product
- SKU:
- N0580-09A1
- Application:
- Western Blot
- translate.label.attr.clone:
- 11n135
- Clonality:
- Monoclonal
- Extra Details:
- NEDD9 (also HEF1 and CASL) is a 93kD (predicted) member of the CAS family of proteins. It is widely expressed, and serves to integrate integrin b1 and BCR signaling with cell mitotic pathways. Human NEDD9 is 834aa in length. It contains one SH3 domain (aa3-65), a Tyr-rich SH2-binding region (aa90-350), one Ser-rich four a-helix bundle protein interaction site (aa350-650), and an HLH motif that mediates Cas protein dimerization (aa710-760). NEDD9 usually runs at 105-115kD in SDS-PAGE due to phosphorylation. It is also cleaved near Leu361 to generate an active N-terminal 55kD fragment. One isoform shows a deletion of aa155-188, while two others show 20-7aa substitutions for aa155-834, 1-153, respectively. Over aa153-366, human and mouse NEDD9 share 85aa identity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1ug/ml with lysates of Daudi human Burkitt's lymphoma cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Lys153-Asn366 from human NEDD9 expressed in E. coli.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NEDD9. Does not react with recombinant human NEDD4 or NEDD8.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
