NCX3 (Sodium Calcium Exchanger) (Not for Export EU)
Product Sizes
100ul
£704.00
N0569-50-100UL
About this Product
- SKU:
- N0569-50
- Clonality:
- Polyclonal
- Extra Details:
- Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein. NCX1 (rat 971aa, human 970aa, mouse 970aa) is most prominently expressed in the heart where it plays a major role in excitation-contraction coupling, but is also present in most other tissues. Alternative spicing of NCX1/NACA1 produces numerous tissue specific isoforms (heart NACA1; Kidney NACA2, -3, and -7; brain NACA4-6). NCX2 (rat 921aa) is restricted to brain and skeletal muscle. NCX3 (rat 927aa) shares 73-75% with NCX1 and NCX2. NCX3 is also restricted to brain and muscle. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000 (ECL). ELISA: 1:10,000-1:50,000. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: N0569-30: NCX3, Rat, Control Peptide (Sodium Calcium Exchanger) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 20aa from rat NCX3 conjugated to KLH. Does not have significant sequence homology with other NCX. Species Sequence Homology: mouse; 90%, human; 80%.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat NCX3 (Sodium Calcium Exchanger). Epitope: N-terminal, extracellular domain.
- Source:
- rat
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
