NaPi-2A (Npt IIa) (Not for Export EU)
Product Sizes
100ul
£704.00
N0035-24A-100UL
About this Product
- SKU:
- N0035-24A
- Clonality:
- Polyclonal
- Extra Details:
- Inorganic phosphate (Pi) levels are hormonally regulated that affects the physiological activity of bone, kidney, and small intestine. At least 4 groups of structurally and functionally related proteins are in involved in Pi transport: Type I-related NaPi transporters designated NPT1, Npt1 and NaPi-1 respectively in humans, mouse and rabbit are expressed in the kidney and liver. Its expression and activity are not regulated by Pi deprivation or parathyroid hormone (PTH) and its role in Pi-homeostasis is not clear. Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opossum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal and tubular Pi reabsorption. Type IIb, designated as NaPi-IIB or NaPi-2b in rat/mouse, and NaPi-3b in human, is closely related isoform of the NaPi-2 family. It is expressed in small intestine and lung. Type III NaPi transporters, originally described as a family of cell surface receptors for gibbon ape leukemia virus (GALV) and murine amphotropic retrovirus (A-MuLv), share very low (<20%) sequence homology with Type I and II proteins, and are found in most tissues. Human homologs are now designated as PiT1 and PiT2. Pit1 and Pit2 are ~62% related and predicted to contain 10 TM domains that is in contrast with Type 1/2 transporters (6-8 TM). The N and C-termini are predicted to be cytoplasmic. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000; Under reducing conditions 2 bands of ~40-50kD are observed. May also form multimeric structures (170, 200kD). ELISA: 1:10,000-50,000. Control peptide (N0035-24G) can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 21aa within the 1st extracellular loop (between TM1-2) of human NPT2a (aa126-146), conjugated tp KLH. Species Sequence Homology: rat, mouse, sheep, opposum; 100%, rabbit; 95%, zebra fish; 85%, frog; 71%.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NaPi-2A. The sequence also has significant sequence homology (~65%) with mouse, rat and human Type-IIb transporters.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
