Mycobacterium tuberculosis CFP10 (APC)
Product Sizes
100ul
£1157.00
M9699-59A-APC-100UL
About this Product
- SKU:
- M9699-59A-APC
- Application:
- FLISA
- translate.label.attr.clone:
- 9B93
- Clonality:
- Monoclonal
- Extra Details:
- Mycobacterium tuberculosis is the most common cause of tuberculosis. Primary infection begins with inhalation of 1 to 10 aerosolised bacilli. The pathogenicity of the organism is determined by its ability to escape host immune responses as well as eliciting delayed hypersensitivity. Alveolar macrophages engulf the invading cells but are unable to mount an effective defense. Several virulence factors are responsible for this apparent failure; most notably in the mycobacterial cell wall are the cord factor, lipoarabinomannan, and the 65kD heat shock protein or HSP65. The emergence of new strains of resistant Mycobacterium tuberculosis has created new interest in clinical diagnosis. Studies have shown immunohistochemical techniques to be superior to conventional special stains. Thus the demonstration of mycobacterial antigens are not only useful in establishing mycobacterial aetiology, but can also be used as an alternative method to the conventional Ziehl-Neelsen method. Applications: Suitable for use in FLISA. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from BALB/c mice Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Allophycocyanin (APC) is light sensitive. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to CFP10 Mycobacterium tuberculosis H37RV.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Purity:
- Purified by Protein G affinity chromatography from ascites (≥95% pure).
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes the CFP antigen of Mycobacterium tuberculosis.
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Monoclonal Antibody
