Mucin 16 (MUC16, MUC-16, CA125, CA-125, Cancer Antigen 125, FLJ14303, Mucin 16 Cell Surface Associated, Cancer-related Tumor Marker CA125, Ovarian Carcinoma Antigen CA125)
Product Sizes
100ug
£889.00
M4701-95Q-100UG
About this Product
- SKU:
- M4701-95Q
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- MUC-1 (Mucin-1; also episialin, MAM-6, PEM and PUM) is a 150-450kD type I transmembrane glycoprotein that belongs to the Mucin family of molecules. It forms a protective barrier against microbes on the apical surface of most glandular epithelium, and becomes prominently displayed on tumor cells. Mature human MUC-1 is 1232aa in length. It contains an 1135aa extracellular domain (ECD) (aa24-1158), and a signal transducing 73aa cytoplasmic tail (aa1182-1255). The ECD is highly polymorphic as evidenced by a highly variable number of 20 aa O-glycosylated tandem repeats. Intracellular processing cleaves MUC-1 between Gly1097 and Ser1098, generating a noncovalently linked heterodimer that is expressed on the cell surface. The C-terminus is 22-25kD in size, while the N-terminus is highly variable (70-400kD in size), a function of the number of resident tandem repeats. Cell surface processing by TACE releases a soluble ECD fragment of variable MW. There are multiple splice variants that show massive reductions in the size of the ECD. Over (aa126-145), human MUC-1 shares < 50% aa identity with mouse MUC-1. When compared to human, mouse MUC-1 is found not to be polymorphic and possess poorly conserved tandem repeat sequences. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 5-15ug/ml using immersion fixed paraffin-embedded sections of human breast Western Blot: 1ug/ml using human lung tissue Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Pro126-Arg145 from human MUC-1, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human MUC-1. Crossreactivity: <5% recombinant human MUC-16.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
