MTH1 [KO Validated]
Product Sizes
200ul
£685.00
M4695-200UL
About this Product
- SKU:
- M4695
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- Clonality:
- Polyclonal
- Extra Details:
- Oxygen radicals damage chromosomal DNA causing cell death and inducing mutations. Among the various classes of DNA damage caused by oxygen radicals, an oxidized form of guanine base (8-oxoguanine) appears to be important as it can pair with cytosine and adenine and G:C to T:A transversion mutation occurs. A significant amount of 8-OxoG is formed in the chromosomal DNA of mammalian cells, with most damaged nucleotides excised from the DNA and excreted in the urine. Along with 8-oxoG being present in oxidatively damaged DNA, 8-oxo-deoxyguanosine (8-oxo-dGTP) is formed in the nucleotide pool during normal cellular metabolism and following oxidative stress. The 8-oxo-dGTP nucleotide can be incorporated in DNA during polymerization and can result in a mispairing unless repaired. MTH converts 8-oxo-dGTP in the nucleotide pool to the monophosphate and prevents the misincorporation of 8-oxo-dGTP into DNA. MTH also recognizes 8-oxo-rGTP, which could incorporate into RNA during gene transcription leading to missense or nonsense protein production. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Knockout Validated. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:500 Immunohistochemistry (Frozen): 1:200 Immunohistochemistry: Paraffin sections Immunofluorescence (IC): 1:50-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- A C-terminal peptide derived from the human MTH1 conjugated to KLH. MW:18kD.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
- Purity:
- Purified by Immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human MTH1 at 18kD. Species Crossreactivity: mouse and rat
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
