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Antibodies

Mrc2 (Macrophage Mannose Receptor 2, uPARAP, Endo180)

Product Sizes
100ug
£876.00
M4685-94-100UG
About this Product
SKU:
M4685-94
Application:
Western Blot
Clonality:
Polyclonal
Extra Details:
Mrc2 (macrophage mannose receptor 2; also uPARAP/Endo180) is a 180kD member of the mannose receptor family. It is found on fibroblasts, macrophages and endothelial cells. Mrc2 binds and internalizes glycoproteins plus collagen types I and IV. Mouse Mrc2 is a type I transmembrane glycoprotein that is 1479 amino acids (aa) in length. It possesses a 1383 aa extracellular domain (ECD) (aa 31-1479) and a short 45 aa cytoplasmic tail. The ECD contains eight C-type lectin domains plus one fibronectin type II domain (aa 181-229) that binds collagen. There are two splice variants, one which shows an eight aa substitution for aa 173-1479, and a second that shows a 72 aa substitution for aa 982-1479. Over aa 31-1413, mouse Mrc2 shares 91% and 97% aa identity with human and rat Mrc2, respectively. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rmMrc2 is ~ 1ng/well. Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Sheep
Immunogen:
NS0-derived rmMrc2 extracellular domain. (aa 31 - 1413)
Isotype:
IgG
Physical State:
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Purity:
Purified by immunoaffinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse Mrc2.
Source:
mouse
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Polyclonal Antibody